Xenotransplantation

Zebrafish, as a model organism, are an important tool for studying human diseases due to their transparent embryos, rapid development, and genome highly similar to humans. Xenotransplantation technology allows cell lines, tumor cells, etc., from humans or other species to be transplanted into zebrafish, providing a living platform to observe cell behavior, tumor growth, angiogenesis, and drug responses.

---

Technical process:

1. Cell preparation:Select and label the cells to be transplanted (such as human cancer cells) for tracking purposes.
2. Embryo processing:Zebrafish embryos were cultured to the appropriate stage and then transferred two days after fertilization.
3. Cell injection:Cells are injected into specific sites in zebrafish embryos using microinjection techniques.
4. Observation and Analysis:Fluorescence microscopy was used to track the survival, proliferation, migration, and interaction of transplanted cells with host tissues.
5. Drug testing:Candidate drugs were added to the transplantation model to assess their effects on transplanted cells and to conduct efficacy and toxicity analyses.

Application scope:

• Cancer research:Observe the growth and metastasis behavior of tumor cells in vivo.
• Drug development:To evaluate the efficacy and safety of new drugs on human cells.