質體訂購-國衛院分支

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5’ entry clonesp5E-hsp70 Lawson Lab 1.5kb hsp70 promoter for heat-shock inductionp5E-MCS Lawson Lab multiple-cloning site from pBluescriptp5E-bactin2 Lawson Lab 5.3kb beta-actin promoter (ubiquitous)p5E-UAS Lawson Lab 10X UAS element and basal promoter for Gal4 responsep5E-h2afx Lawson Lab 1kb histone2A-X promoter (quasi-ubiquitou)p5E-Fse-Asc Lawson Lab restriction sites for 8-cutters FesI and AscIp5E-CMV/SP6 Lawson Lab 1kb CMV/SP6 cassette from pCS2+p5E-FA-bas Kwan Lab FseI/AscI sites followed by the adenovirus E1B TATA boxp5E-FA-gata2bas Kwan Lab FseI/AscI sites followed by the gata2 basal promoter

Tol2 transposase vectorpCS2FA-transposase Koichi Kawakami for in vitro transcription of capped Tol2 transposase mRNApT3TS-SB11 Kwan Lab Sleeping Beauty transposase plasmid

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質體訂購-國衛院分支

TZCF@NHRI現有質體

5’ entry clones

p5E-hsp70 Lawson Lab 1.5kb hsp70 promoter for heat-shock induction

p5E-MCS Lawson Lab multiple-cloning site from pBluescript

p5E-bactin2 Lawson Lab 5.3kb beta-actin promoter (ubiquitous)

p5E-UAS Lawson Lab 10X UAS element and basal promoter for Gal4 response

p5E-h2afx Lawson Lab 1kb histone2A-X promoter (quasi-ubiquitou)

p5E-Fse-Asc Lawson Lab restriction sites for 8-cutters FesI and AscI

p5E-CMV/SP6 Lawson Lab 1kb CMV/SP6 cassette from pCS2+

p5E-FA-bas Kwan Lab FseI/AscI sites followed by the adenovirus E1B TATA box

p5E-FA-gata2bas Kwan Lab FseI/AscI sites followed by the gata2 basal promoter

Middle entry clones

pME-nlsmCherry Lawson Lab nuclear-localized mCherry

pME-H2AmCherry Lawson Lab mCherry fused to zebrafisf histone H2A.F/Z

pME-MCS Lawson Lab multiple-cloning site from pBluescript

pME-EGFP Lawson Lab EGFP

pME-EGFPCAAX Lawson Lab membrane-localized (prenylated) EGFP; fused to 21 aa of H-ras

pME-nlsEGFP Lawson Lab nuclear-localized EGFP

pME-mCherry Lawson Lab monomeric red fluorophore mCherry

pME-Gal4VP16 Lawson Lab Gal4 DNA binding domain fused to VP16 transactivation domain

pME-mCherryCAAX Lawson Lab prenylated mCherry

pME-EGFP no stop Lawson Lab EGFP, no stop (to make N-terminal fusions)

pME-mCherry no stop Lawson Lab mCherry, no stop (to make N-terminal fusions)

pME-mApple Kwan Lab mApple

pME-mAppleCAAX Kwan Lab membrane-localized (prenylated) mApple; fused to 21 aa of H-ras

pME-nlsmApple Kwan Lab nuclear-localized mApple

pME-Cerulean Kwan Lab Cerulean

pME-CeruleanCAAX Kwan Lab membrance-localized (prenylated) Cerulean

pME-nlsCerulean Kwan Lab nuclear-localized Cerulean

pME-emGFP Kwan Lab emerald GFP; monomerized (A207K mutation)

pME-emGFP no stop Kwan Lab emerald GFP (A207K), flanked by FseI/AscI, fallowed by linker

pME-Gal4 Kwan Lab native Gal4

pME-HA-Gal4 Kwan Lab HA-tagged native Gal4

pME-Gal4FF Kwan Lab Gal4 DNA binding domain fused to two tx activation modules of VP16; less toxic than Gal4VP16

pME-iCre Kwan Lab Cre

pME-CreERT2 Kwan Lab Cre fused to a mutant estrogen ligand-binding domain (ERT2)

pME-loxP-stop-loxP Kwan Lab stop sequence from pBS302 flanked by loxP sites

pME-loxP-mCherry-stop-loxP Kwan Lab mCherry followed by stop sequence from pBS302 flanked by loxP sites

3’ entry clones

p3E-MTpA Lawson Lab 6x myc tag for C-terminal fusuions, plus SV40 late polyA

p3E-polyA Lawson Lab SV40 late polyA signal

p3E-EGFPpA Lawson Lab EGFP for C-terminal fusions, plus SV40 late polyA

p3E-mcherrypA Lawson Lab mCherry for C-terminal fusions, plus SV40 late polyA

p3E-IRES-EGFPpA Lawson Lab IRES driving EGFP plus SV40 late polyA

p3E-IRES-EGFPCAAXpA Lawson Lab IRES driving prenylated EGFP plus SV40 late polyA

p3E-IRES-nlsEGFPpA Lawson Lab IRES driving nuclear EGFP plus SV40 late polyA

p3E-linker-emGFP-pA Kwan Lab linker followed by em GFP (A207K), flanked by FseI/AscI (for fusions)

p3E-2A-EGFPpA Kwan Lab PTV-1 2A peptide followed by EGFP plus late SV40 polyA

p3E-2A-EGFPCAAXpA Kwan Lab PTV-1 2A peptide followed by EGFPCAAX plus late SV40 polyA

p3E-2A-nlsEGFPpA Kwan Lab PTV-1 2A peptide followed by nlsEGFP plus late SV40 polyA

p3E-2A-mCherrypA Kwan Lab PTV-1 2A peptide followed by mCherry plus late SV40 polyA

p3E-2A-mCherryCAAXpA Kwan Lab PTV-1 2A peptide followed by mCherryCAAX plus late SV40 polyA

p3E-2A-nlsmCherrypA Kwan Lab PTV-1 2A peptide followed by nlsmCherry plus late SV40 polyA

p3E-2A-pCS2MCS-pA Kwan Lab PTV-1 2A peptide followed by cloning site from pCS2 plus late SV40 polyA

Destiny vectors

pDestTol2pA2 Lawson Lab pDestTol2pA with ~2 kb extraneous sequence removed

pDestTol2CG2 Lawson Lab pDestTol2CG2 with ~2 kb extraneous sequence removed

pDestTol2pA3 Kwan Lab pDestTol2pA2 with I-SceI sites and miniTol2 inverted repeats

pDestTol2pA4 Kwan Lab pDestTol2pA3 with unique sites added

pDestTol2pA5 Kwan Lab pDestTol2pA4 with insulators

pDestTol2pA6 Kwan Lab pDestTol2pA2 (Kawakami Tol2 inberted repeats) with unique sites added

pDestTol2pA7 Kwan Lab pDestTol2pA6 with insulators

pDestTol2CG3 Kwan Lab pDestTol2pA3 with cmlc2:GFP transgenesis maker

pDestTol2CG4 Kwan Lab pDestTol2pA4 with cmlc2:GFP transgenesis maker

pDestTol2CG5 Kwan Lab pDestTol2pA5 with cmlc2:GFP transgenesis maker

pDestSBpA Kwan Lab attR4-R3 gate w/ SV40 polyA flanked by sleeping Beauty inverted repeats

pDestSBCG Kwan Lab pDestSBpA with cmlc2:GFP transgenesis maker

Tol2 transposase vector

pCS2FA-transposase Koichi Kawakami for in vitro transcription of capped Tol2 transposase mRNA

pT3TS-SB11 Kwan Lab Sleeping Beauty transposase plasmid

Plasmids deposited by Dr. 鄭邑荃

her1

her2

her3

her5

her6

her7

her8a

her9