{"id":618,"date":"2026-09-03T14:52:25","date_gmt":"2026-09-03T06:52:25","guid":{"rendered":"https:\/\/tzcf.org\/?page_id=618"},"modified":"2026-09-08T09:27:47","modified_gmt":"2026-09-08T01:27:47","slug":"tg236","status":"publish","type":"page","link":"https:\/\/tzcf.org\/en\/tg236\/","title":{"rendered":"TG236"},"content":{"rendered":"<div class=\"wp-block-group alignfull has-accent-2-background-color has-background has-medium-font-size has-global-padding is-layout-constrained wp-container-core-group-is-layout-c167fab6 wp-block-group-is-layout-constrained\" style=\"margin-top:0;margin-bottom:0;padding-top:0;padding-right:var(--wp--preset--spacing--50);padding-bottom:0;padding-left:var(--wp--preset--spacing--50)\">\n<h1 class=\"wp-block-heading has-text-align-center alignwide has-text-color has-link-color wp-elements-1\" style=\"color:#1aa2cb\"><strong>Acute toxicity test of zebrafish embryos (OECD TG 236)<\/strong><\/h1>\n\n\n\n<hr class=\"wp-block-separator alignwide has-alpha-channel-opacity is-style-wide\" style=\"margin-top:var(--wp--preset--spacing--10);margin-bottom:var(--wp--preset--spacing--10)\"\/>\n\n\n\n<div class=\"wp-block-columns alignwide is-layout-flex wp-container-core-columns-is-layout-3a88641f wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:109.3%\">\n<p class=\"has-heading-font-family has-large-font-size wp-block-paragraph\" style=\"line-height:1.4\">The use of zebrafish embryos as an alternative to toxicity detection has been practiced in EU countries for many years, and several related toxicity testing methods have been published (such as OECD-committed TG236 and TG234). This platform, in order to promote alternatives to animal testing (the 3R principle) and assist governments in providing effective biodetection methods for various toxic substances related to daily life (such as those affecting the environment, agricultural products, and wastewater), has specifically added zebrafish embryo toxicity detection services. This service is based on the OECD-committed standard test method \u2013 TG 236 \u2013 and is used to test the toxicity of chemicals after 96 or 120 hours of immersion, providing an alternative to laboratory animals for environmental pollution, drug screening, and food safety testing.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><br><span style=\"color: #C62828\"><strong>The OECD TG-236 testing method can provide the following test report content:<\/strong><\/span><\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li class=\"has-text-color has-link-color wp-elements-2\" style=\"color:#c62828\"><strong>Experimental results (survival rate and LC value)<\/strong><\/li>\n\n\n\n<li class=\"has-text-color has-link-color wp-elements-3\" style=\"color:#c62828\"><strong>The fish embryo survival rate curves for different drug concentrations are shown in the figure below.<\/strong><\/li>\n\n\n\n<li class=\"has-text-color has-link-color wp-elements-4\" style=\"color:#c62828\"><strong>The survival rate data of fish embryos at different time points for various drug concentrations can be found in the following figure.<\/strong><\/li>\n\n\n\n<li class=\"has-text-color has-link-color wp-elements-5\" style=\"color:#c62828\"><strong>Toxrat Analysis Report (one copy)<\/strong><\/li>\n<\/ol>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:var(--wp--preset--spacing--40)\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns alignwide is-layout-flex wp-container-core-columns-is-layout-3a88641f wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<figure class=\"wp-block-image alignwide size-full is-style-rounded is-style-rounded--1\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" width=\"406\" height=\"254\" data-attachment-id=\"655\" data-permalink=\"https:\/\/tzcf.org\/en\/tg236\/oecd-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-2.jpg?fit=406%2C254&amp;ssl=1\" data-orig-size=\"406,254\" data-comments-opened=\"1\" data-image-title=\"oecd-2\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-2.jpg?fit=406%2C254&amp;ssl=1\" src=\"https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-2.jpg?resize=406%2C254&#038;ssl=1\" alt=\"\" class=\"wp-image-655\" srcset=\"https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-2.jpg?w=406&amp;ssl=1 406w, https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-2.jpg?resize=300%2C188&amp;ssl=1 300w\" sizes=\"auto, (max-width: 406px) 100vw, 406px\" \/><figcaption class=\"wp-element-caption\">\n        Fish embryo survival rate curve\n    <\/figcaption><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:50%\">\n<figure class=\"wp-block-image size-full is-resized is-style-rounded is-style-rounded--2\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" width=\"406\" height=\"254\" data-attachment-id=\"656\" data-permalink=\"https:\/\/tzcf.org\/en\/tg236\/oecd-1\/\" data-orig-file=\"https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-1.jpg?fit=406%2C254&amp;ssl=1\" data-orig-size=\"406,254\" data-comments-opened=\"1\" data-image-title=\"oecd-1\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-1.jpg?fit=406%2C254&amp;ssl=1\" src=\"https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-1.jpg?resize=406%2C254&#038;ssl=1\" alt=\"\" class=\"wp-image-656\" style=\"aspect-ratio:1.5984620116887112;width:630px;height:auto\" srcset=\"https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-1.jpg?w=406&amp;ssl=1 406w, https:\/\/i0.wp.com\/tzcf.org\/wp-content\/uploads\/2026\/09\/oecd-1.jpg?resize=300%2C188&amp;ssl=1 300w\" sizes=\"auto, (max-width: 406px) 100vw, 406px\" \/><figcaption class=\"wp-element-caption\">Survival rate of fish embryos at different time points<\/figcaption><\/figure>\n<\/div>\n<\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>\u6591\u99ac\u9b5a\u80da\u80ce\u6025\u6bd2\u6027\u6e2c\u8a66 (OECD TG 236) \u5229\u7528\u6591\u99ac\u9b5a\u80da\u80ce\u4f5c\u70ba\u6bd2\u6027\u5075\u6e2c\u7684\u66ff\u4ee3\u65b9\u6848\u5728\u6b50\u76df\u570b\u5bb6\u5df2\u884c\u4e4b\u591a\u5e74\uff0c\u4e26 [&hellip;]<\/p>\n","protected":false},"author":282380303,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"jetpack_seo_schema_type":"","_wpcom_ai_launchpad_about_page":false,"_wpcom_ai_launchpad_gallery_page":false,"_wpcom_ai_launchpad_contact_page":false,"_wpcom_ai_launchpad_events_page":false,"_wpcom_ai_launchpad_video_page":false,"_wpcom_ai_launchpad_portfolio_piece":false,"_jetpack_hide_featured_image":false,"_jetpack_featured_image_duplicate_dismissed":0,"footnotes":""},"class_list":["post-618","page","type-page","status-publish","hentry"],"jetpack_likes_enabled":true,"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/PhjQhi-9Y","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/tzcf.org\/en\/wp-json\/wp\/v2\/pages\/618","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/tzcf.org\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/tzcf.org\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/tzcf.org\/en\/wp-json\/wp\/v2\/users\/282380303"}],"replies":[{"embeddable":true,"href":"https:\/\/tzcf.org\/en\/wp-json\/wp\/v2\/comments?post=618"}],"version-history":[{"count":11,"href":"https:\/\/tzcf.org\/en\/wp-json\/wp\/v2\/pages\/618\/revisions"}],"predecessor-version":[{"id":678,"href":"https:\/\/tzcf.org\/en\/wp-json\/wp\/v2\/pages\/618\/revisions\/678"}],"wp:attachment":[{"href":"https:\/\/tzcf.org\/en\/wp-json\/wp\/v2\/media?parent=618"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}